Answer: There are mainly two types of such situations. One is when there are problems during slicing, such as relatively hard tissue containing calcified tissue, or when the cutting edge is not sharp enough during slicing, resulting in wrinkles; When the wax block is not completely cooled, its texture is relatively soft and it is still prone to wrinkles during slicing. Another situation is that the water temperature is not enough during the rinsing process, and the cut tissue slices cannot be fully unfolded during rinsing, which can also lead to the formation of wrinkles.

Solution: The blade is not sharp enough and needs to be replaced. The water temperature of the float generally needs to be 10 ℃ lower than the melting point of paraffin, and the float time needs to be adjusted to ensure that the slices are fully stretched. If partial calcification occurs in the original tissue, it is difficult to avoid.

Answer: There are mainly two types of such situations. One is that the water temperature is too high during the float, which leads to the scattering of pathological sections and further rupture; Another situation is due to the high fat content in the sliced tissue itself, which is not completely dehydrated, especially in warm water, making it easier for the fat to disperse.

Solution: It is necessary to adjust the water temperature of the float in a timely manner, so that the water temperature can be suitable for the flat development of the slice float, but it will not overheat and cause expansion and rupture. In contrast, when there is a high content of adipose tissue in the slice, the float time needs to be shortened. If the slice is prone to rupture, it can also ensure tissue integrity and avoid rinsing.

Answer: This type of situation is mainly due to the presence of hard texture components in the tissue during tissue sectioning, which causes the slicing knife to break out, and under the microscope, a defect pattern similar to a long road will appear in the tissue sectioning.

Solution: In most cases, the knife position needs to be replaced. Similarly, if there are calcified lesions and lymph node tissues in the tissue, these knife marks will become relatively unavoidable. If it does not affect staining observation and pathological diagnosis, it can be appropriately accommodated.

Answer: This type of situation is mainly due to the speed of tissue cutting being too fast and not fully aligned, resulting in an uneven cross-section of the slice. Under the microscope, some large or small tissue voids may appear.

Solution: When cutting tissue slices, it is necessary to control the amplitude of the feed, and it is recommended to set the thickness to 10 for rough trimming μ m. When refining, adjust to make the wax block cut surface smooth, which can reduce the occurrence of empty holes, small white spots, and roughness.

Answer: There are mainly two types of such situations. One is that when embedding the tissue in the embedding box, the tissue is not completely flattened, which will result in not cutting to the maximum section during slicing. The other is that when sampling the tissue, it is not flat, which will also cause missing or omitted slices, which will affect the next step of the experiment.

Solution: Each step of organizational processing needs to be operated in a standardized manner as much as possible. When taking materials, the cutting surface needs to be flat. When embedding tissues, each piece of tissue needs to be as flat as possible. During each cutting, it is necessary to observe whether the wax block has exposed the maximum cutting surface. If the effect is not ideal, the tissue can be re embedded.

Answer: This type of situation is mainly due to the presence of excess wax blocks in the sample tissue during embedding, which leads to insufficient tightness between the entire embedding box and the tissue, resulting in some hollow areas, leading to different areas of too deep or too shallow staining regret.

Solution: In such cases, it is necessary to first check whether the slicing instrument is completely clamped, and also check whether the slicing machine tool holder is misaligned. If the wax block is not firmly or even hollow due to insufficient embedding, it needs to be re embedded in the tissue. When encountering situations where the organization is relatively hard, it is necessary to reduce the rotational speed appropriately, let the wax block warm up appropriately, and then re slice.

Answer: This type of situation usually occurs in the sample tissue, including the inner membrane of organs and some lymphoid tissues. The reasons for this situation will be mainly reflected in the fact that the temperature of the wax block slice is lower than the expected temperature, resulting in relatively brittle tissue. Failure to control the speed during slicing and slicing too quickly can also lead to slice fragmentation.

Solution: When slicing, adjust the operating table temperature to 0 ℃ for slicing, while reducing the slicing speed, which can effectively reduce the occurrence of cracks and lines. If conditions permit, ice blocks at 0 ℃ can also be used as cooling media for wax blocks, which can effectively reduce such situations.

Solution: In such cases, the first consideration is the freshness of the tissue. We recommend that the tissue be cut open and fixed within half an hour of being detached. The fixation time should be controlled between 8-16 hours based on the size of the specimen. It is recommended that the specimen volume should not exceed 1 cm3, and the specific dehydration situation can be determined by the experiment.

Answer: This type of situation is mainly manifested in the wax block being left for a certain period of time, which will cause the section to expand and the surface to turn white, leading to the scrapping of the slices. Perhaps due to incomplete dehydration of the tissue, some water may undergo reverse osmosis.

Solution: This type of situation is mostly due to the wax block being left on the melted ice surface for too long. You can place the wax block on the ice surface for cooling a few minutes before slicing, which can greatly avoid this situation.

Answer: This type of situation is mainly due to the rapid temperature drop of the wax block during cooling, and the internal paraffin has not been completely cooled yet. If the module is pried open directly in this situation, it will cause the wax block to crack.

Solution: It is necessary to analyze and solve the problem from two aspects. The first is to check the quality of the paraffin. If the inspection is a quality issue, the product needs to be replaced. The second is not to directly cool the paraffin quickly during cooling. Try to let the paraffin cool naturally and completely solidify before further separation, which can effectively reduce the occurrence of such situations.